Title | Multi-centre evaluation of mass spectrometric identification of anaerobic bacteria using the VITEK® MS system. |
Publication Type | Journal Article |
Year of Publication | 2014 |
Authors | Garner O, Mochon A, Branda J, Burnham C-A, Bythrow M, Ferraro M, Ginocchio C, Jennemann R, Manji R, Procop GW, Richter S, Rychert J, Sercia L, Westblade L, Lewinski M |
Journal | Clin Microbiol Infect |
Volume | 20 |
Issue | 4 |
Pagination | 335-9 |
Date Published | 2014 Apr |
ISSN | 1469-0691 |
Keywords | Bacteria, Anaerobic, Bacterial Infections, Bacteriological Techniques, Humans, Sensitivity and Specificity, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization |
Abstract | Accurate and timely identification of anaerobic bacteria is critical to successful treatment. Classic phenotypic methods for identification require long turnaround times and can exhibit poor species level identification. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is an identification method that can provide rapid identification of anaerobes. We present a multi-centre study assessing the clinical performance of the VITEK(®) MS in the identification of anaerobic bacteria. Five different test sites analysed a collection of 651 unique anaerobic isolates comprising 11 different genera. Multiple species were included for several of the genera. Briefly, anaerobic isolates were applied directly to a well of a target plate. Matrix solution (α-cyano-4-hydroxycinnamic acid) was added and allowed to dry. Mass spectra results were generated with the VITEK(®) MS, and the comparative spectral analysis and organism identification were determined using the VITEK(®) MS database 2.0. Results were confirmed by 16S rRNA gene sequencing. Of the 651 isolates analysed, 91.2% (594/651) exhibited the correct species identification. An additional eight isolates were correctly identified to genus level, raising the rate of identification to 92.5%. Genus-level identification consisted of Actinomyces, Bacteroides and Prevotella species. Fusobacterium nucleatum, Actinomyces neuii and Bacteroides uniformis were notable for an increased percentage of no-identification results compared with the other anaerobes tested. VITEK(®) MS identification of clinically relevant anaerobes is highly accurate and represents a dramatic improvement over other phenotypic methods in accuracy and turnaround time. |
DOI | 10.1111/1469-0691.12317 |
Alternate Journal | Clin Microbiol Infect |
PubMed ID | 23927597 |
Related Faculty:
Lars Westblade, Ph.D.