Title | Long-term in vivo biocompatibility of single-walled carbon nanotubes. |
Publication Type | Journal Article |
Year of Publication | 2020 |
Authors | Galassi TV, Antman-Passig M, Yaari Z, Jessurun J, Schwartz RE, Heller DA |
Journal | PLoS One |
Volume | 15 |
Issue | 5 |
Pagination | e0226791 |
Date Published | 2020 |
ISSN | 1932-6203 |
Keywords | Animals, Biocompatible Materials, Biomarkers, DNA, Single-Stranded, Endosomes, Humans, Liver, Lung, Mice, Nanotechnology, Nanotubes, Carbon, Optical Imaging, Spleen, Tissue Distribution |
Abstract | Over the past two decades, measurements of carbon nanotube toxicity and biodistribution have yielded a wide range of results. Properties such as nanotube type (single-walled vs. multi-walled), purity, length, aggregation state, and functionalization, as well as route of administration, greatly affect both the biocompatibility and biodistribution of carbon nanotubes. These differences suggest that generalizable conclusions may be elusive and that studies must be material- and application-specific. Here, we assess the short- and long-term biodistribution and biocompatibility of a single-chirality DNA-encapsulated single-walled carbon nanotube complex upon intravenous administration that was previously shown to function as an in-vivo reporter of endolysosomal lipid accumulation. Regarding biodistribution and fate, we found bulk specificity to the liver and >90% signal attenuation by 14 days in mice. Using near-infrared hyperspectral microscopy to measure single nanotubes, we found low-level, long-term persistence in organs such as the heart, liver, lung, kidney, and spleen. Measurements of histology, animal weight, complete blood count; biomarkers of organ function all suggest short- and long-term biocompatibility. This work suggests that carbon nanotubes can be used as preclinical research tools in-vivo without affecting acute or long-term health. |
DOI | 10.1371/journal.pone.0226791 |
Alternate Journal | PLoS One |
PubMed ID | 32374764 |
PubMed Central ID | PMC7202660 |
Grant List | DP2 HD075698 / HD / NICHD NIH HHS / United States P30 CA008748 / CA / NCI NIH HHS / United States K08 DK101754 / DK / NIDDK NIH HHS / United States R01 CA234614 / CA / NCI NIH HHS / United States R03 DK117252 / DK / NIDDK NIH HHS / United States R01 DK114321 / DK / NIDDK NIH HHS / United States UL1 TR002384 / TR / NCATS NIH HHS / United States R01 CA215719 / CA / NCI NIH HHS / United States |
Related Faculty:
Jose Jessurun, M.D.