Localization of surface vWF on resting and stimulated platelets.

TitleLocalization of surface vWF on resting and stimulated platelets.
Publication TypeJournal Article
Year of Publication1987
AuthorsRand JH, Gordon RE, Uson RR, Potter BJ
Date Published1987 Nov
KeywordsAdenosine Diphosphate, Blood Platelets, Cell Membrane, Humans, Microscopy, Electron, Platelet Aggregation, Platelet Membrane Glycoproteins, Receptors, Cell Surface, Reference Values, Ristocetin, von Willebrand Factor

We used immunoelectron microscopic localization techniques to investigate whether platelets stimulated by ADP or ristocetin in the plasma milieu bind von Willebrand factor (vWF) to their surfaces. We found by both peroxidase- and ferritin-based methods that unstimulated platelets lack vWF on their surfaces, whereas platelets that are stimulated with ADP or ristocetin have vWF associated with their surfaces. The specificity of the findings was confirmed by absorption studies using severe von Willebrand disease (vWD) and hemophilic plasmas. The anti-vWF antibodies were blocked by incubation with hemophilic plasma but not by incubation with severe vWD plasma. Thus, in the plasma environment, in the presence of fibrinogen, vWF becomes associated with the platelet surface subsequent to stimulation with ADP or ristocetin.

Alternate JournalBlood
PubMed ID2822169
Grant ListHL-17430 / HL / NHLBI NIH HHS / United States
HL-32200 / HL / NHLBI NIH HHS / United States
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Jacob H. Rand, M.D.

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