| Title | Interleukin 6 enhances a cellular activity that functionally substitutes for E1A protein in transactivation. |
| Publication Type | Journal Article |
| Year of Publication | 1991 |
| Authors | Spergel JM, Chen-Kiang S |
| Journal | Proc Natl Acad Sci U S A |
| Volume | 88 |
| Issue | 15 |
| Pagination | 6472-6 |
| Date Published | 1991 Aug 01 |
| ISSN | 0027-8424 |
| Keywords | Adenovirus Early Proteins, Adenoviruses, Human, Base Sequence, Cell Line, Cell Nucleus, DNA Replication, DNA, Neoplasm, DNA, Viral, Fetus, Genes, Viral, HeLa Cells, Humans, Interleukin-6, Liver, Molecular Sequence Data, Oligonucleotide Probes, Oncogene Proteins, Viral, Promoter Regions, Genetic, Restriction Mapping, Transcription Factors, Transcription, Genetic, Transcriptional Activation |
| Abstract | An interleukin 6 (IL-6)-regulated cellular activity in HepG2 cells is found to functionally substitute for the transcriptional transactivator product of the adenovirus transforming gene E1A in transactivating E1A-dependent and E1A-responsive viral early genes. Mutant viruses deficient in E1A expression replicate in HepG2 cells. Induction with IL-6 leads to significant enhancement of synthesis of viral early E1B and E2ae mRNAs by greater than 30-fold and increases viral replication to the wild-type levels. The E1A-substituting activity activates E1A-responsive promoters in transient transfection, and this transcriptional activity is regulated by IL-6 induction. Formation of distinct protein-promoter complexes by binding of proteins in nuclear extracts prepared from HepG2 cells to the E1A-dependent E2ae promoter further supports the possibility that this activity may be a nuclear component in the IL-6 signal transduction pathway. |
| DOI | 10.1073/pnas.88.15.6472 |
| Alternate Journal | Proc Natl Acad Sci U S A |
| PubMed ID | 1830663 |
| PubMed Central ID | PMC52107 |
| Grant List | AI 24404 / AI / NIAID NIH HHS / United States |
Related Faculty:
Selina Chen-Kiang, Ph.D.