Integrated DNA copy number and methylation profiling of lymphoid neoplasms using a single array.

TitleIntegrated DNA copy number and methylation profiling of lymphoid neoplasms using a single array.
Publication TypeJournal Article
Year of Publication2012
AuthorsKwee I, Rinaldi A, Rancoita P, Rossi D, Capello D, Forconi F, Giuliani N, Piva R, Inghirami G, Gaidano G, Zucca E, Bertoni F
JournalBr J Haematol
Volume156
Issue3
Pagination354-7
Date Published2012 Feb
ISSN1365-2141
KeywordsCpG Islands, DNA Methylation, DNA, Neoplasm, Gene Dosage, Hematopoietic Stem Cell Transplantation, Humans, Leukemia, Lymphoma, Lymphoma, Large B-Cell, Diffuse, Oligonucleotide Array Sequence Analysis, Postoperative Period
Abstract

Changes in DNA copy number (CN) and DNA methylation represent important aberrations for lymphomas and other cancers. Here, for the first time, we show that the Illumina Infinium Methylation (IIM) assay, although not originally designed for CN profiling, is able to estimate CN changes. We compared the IIM CN profiles to those obtained with a standard technique in a series of diffuse large B-cell lymphomas: the profiles showed a high degree of consensus. The demonstration of CN profiling as an additional function of the IIM assay may impact the choice of platform for methylation profiling of haematological and solid tumours.

DOI10.1111/j.1365-2141.2011.08946.x
Alternate JournalBr J Haematol
PubMed ID22118580
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