Heterogeneity of viral IL-6 expression in HHV-8-associated diseases.

TitleHeterogeneity of viral IL-6 expression in HHV-8-associated diseases.
Publication TypeJournal Article
Year of Publication1999
AuthorsCannon JS, Nicholas J, Orenstein JM, Mann RB, Murray PG, Browning PJ, DiGiuseppe JA, Cesarman E, Hayward GS, Ambinder RF
JournalJ Infect Dis
Date Published1999 Sep
KeywordsAdult, Aged, DNA, Viral, Female, Herpesvirus 8, Human, Humans, Immunohistochemistry, In Situ Hybridization, Interleukin-6, Lymphoma, Lymphoma, AIDS-Related, Male, Middle Aged, Open Reading Frames, Retrospective Studies, Sarcoma, Kaposi, Tumor Cells, Cultured, Viral Proteins

In order to characterize the expression of the viral interleukin-6 (vIL-6) homologue in various human herpesvirus 8 (HHV-8)-associated diseases, in situ hybridization and immunohistochemistry were applied to formalin-fixed specimens. These assays showed consistent expression of vIL-6 in primary effusion lymphomas and in a case of human immunodeficiency virus (HIV)-associated lymphadenopathy with a Castleman's disease-like appearance. In contrast, Kaposi's sarcoma specimens showed marked differences among specimens. In a consecutive series of specimens from the Johns Hopkins archives, vIL-6 expression was demonstrated in one of 13 cases. However, among 7 specimens selected from the AIDS Malignancy Bank because of their high levels of the T1.1 lytic transcript and virion production, vIL-6 expression was consistently demonstrated in infiltrating mononuclear cells and occasional spindle-shaped cells. Thus vIL-6 expression in clinical specimens correlates with other measures of the lytic viral cycle. Both assays generally give congruent results and are consistent with the possibility that vIL-6 expression plays a role in the pathogenesis of a variety of HHV-8-associated diseases.

Alternate JournalJ Infect Dis
PubMed ID10438372
Grant ListP030 CA06973 / CA / NCI NIH HHS / United States
P030 CA68485 / CA / NCI NIH HHS / United States
UO1 CA70062 / CA / NCI NIH HHS / United States
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Ethel Cesarman, M.D., Ph.D.

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