Genetic Profiling of African American Patients With Prostatic Adenocarcinoma Metastatic to the Lymph Nodes: A Pilot Study.

TitleGenetic Profiling of African American Patients With Prostatic Adenocarcinoma Metastatic to the Lymph Nodes: A Pilot Study.
Publication TypeJournal Article
Year of Publication2024
AuthorsBidot S, Yin J, Zhou P, Zhang L, Deeb KK, Smith G, Hill CE, Xiu J, Bilen MA, Case KB, Tinsley M, Carthon B, Harik LR
JournalArch Pathol Lab Med
Date Published2024 Mar 01
KeywordsAdenocarcinoma, Androgen Antagonists, Black or African American, Carrier Proteins, DNA Helicases, Humans, Lymph Nodes, Male, Nuclear Proteins, Pilot Projects, Poly-ADP-Ribose Binding Proteins, Prostatic Neoplasms, Receptors, Androgen, Repressor Proteins, Retrospective Studies, RNA Helicases, RNA Recognition Motif Proteins

CONTEXT.—: Genetic profiling data of prostatic adenocarcinoma are derived from predominantly White patients. In African Americans, prostatic adenocarcinoma has a poorer prognosis, raising the possibility of distinct genetic alterations.

OBJECTIVE.—: To investigate the genomic alterations of prostatic adenocarcinoma metastatic to regional lymph nodes in African American patients, with an emphasis on SPOP mutation.

DESIGN.—: We retrospectively reviewed African American patients with pN1 prostatic adenocarcinoma managed with radical prostatectomy and lymph node dissection. Comprehensive molecular profiling was performed, and androgen receptor signaling scores were calculated.

RESULTS.—: Nineteen patients were included. The most frequent genetic alteration was SPOP mutations (5 of 17; 29.4% [95% CI: 10.3-56.0]). While most alterations were associated with a high androgen receptor signaling score, mutant SPOP was exclusively associated with a low median and interquartile range (IQR) androgen receptor signaling score (0.788 [IQR 0.765-0.791] versus 0.835 [IQR 0.828-0.842], P = .003). In mutant SPOP, mRNA expression of SPOP inhibitor G3BP1 and SPOP substrates showed a significantly decreased expression of AR (33.40 [IQR 28.45-36.30] versus 59.53 [IQR 53.10-72.83], P = .01), TRIM24 (3.95 [IQR 3.28-5.03] versus 9.80 [IQR 7.39-11.70], P = .008), and NCOA3 (15.19 [IQR 10.59-15.93] versus 21.88 [IQR 18.41-28.33], P = .046).

CONCLUSIONS.—: African American patients with metastatic prostate adenocarcinoma might have a higher prevalence of mutant SPOP (30%), compared to ∼10% in unselected cohorts with lower expressions of SPOP substrates. In our study, in patients with mutant SPOP, the mutation was associated with decreased SPOP substrate expression and androgen receptor signaling, raising concern for suboptimal efficacy of androgen deprivation therapy in this subset of patients.

Alternate JournalArch Pathol Lab Med
PubMed ID37327205
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Related Faculty: 
Pengbo Zhou, Ph.D.

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