Expression of collagen biosynthetic activities in lymphocytic cells.

TitleExpression of collagen biosynthetic activities in lymphocytic cells.
Publication TypeJournal Article
Year of Publication1978
AuthorsChen-Kiang S, Cardinale GJ, Udenfriend S
JournalProc Natl Acad Sci U S A
Date Published1978 Mar
KeywordsAnimals, Cell Line, Collagen, Cross Reactions, Friend murine leukemia virus, Humans, Leukemia, Experimental, Lymphocyte Activation, Lymphocytes, Mice, Multiple Myeloma, Neoplasms, Experimental, Procollagen-Proline Dioxygenase

Immunoglobulin-producing Merwin plasma cells, MPC-11, have been found to contain proplyl hydroxylase (prolyl-glycyl-peptide,2-oxoglutarate:oxygen oxidoreductase; EC activity and its crossreacting protein, as well as hydroxyproline and a collagenous protein that could not be classified as type I, II, or III collagen. Friend leukemic cells, on the other hand, contained only prolyl hydroxylase. Thymus-derived (T) lymphocytes and bone-marrow-derived (B) lymphocytes freshly isolated from BALB/c mice expressed low but significant prolyl hydroxylase activity. Upon stimulation with phytohemagglutinin, the enzyme activity in T cells increased 22- to 29-fold. Crossreacting protein was also increased and appeared more stable than the prolyl hydroxylase. The effect of lipopolysaccharide stimulation on B cells uas similar but not as pronounced. Thus, even when not accompanied by other collagen biosynthetic activities, prolyl hydroxylase is present in all cells of hematologic origin.

Alternate JournalProc Natl Acad Sci U S A
PubMed ID206896
PubMed Central IDPMC411475
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