Enhancement of cholesterol and cholesteryl ester accumulation in re-endothelialized aorta.

TitleEnhancement of cholesterol and cholesteryl ester accumulation in re-endothelialized aorta.
Publication TypeJournal Article
Year of Publication1980
AuthorsFalcone DJ, Hajjar DP, Minick CR
JournalAm J Pathol
Date Published1980 Apr
KeywordsAnimals, Aorta, Aorta, Abdominal, Aorta, Thoracic, Arteriosclerosis, Cholesterol, Cholesterol Esters, Diet, Endothelium, Fatty Acids, Female, Hypercholesterolemia, Rabbits, Squalene, Triglycerides

The purpose of the experiments reported here was to determine chemically the character and quantity of lipid in re-endothelialized and de-endothelialized areas of rabbit aortas. The aortas of 22 rabbits, Groups I and II, were de-endothelialized with a balloon catheter, and the rabbits were maintained on a lipid-poor diet for 4 weeks. Thirteen rabbits, Group II, were then fed an egg-supplemented diet for 6 weeks. Nine rabbits, Group I, were continued on the lipid-poor diet for an additional 6 weeks. Control rabbits with uninjured aortas were fed a lipid-poor diet for 10 weeks (Group III) or an egg-supplemented diet for 6 weeks (Group IV). Nonesterified cholesterol and fatty acids, cholesteryl esters, triacylglycerols, and squalene were quantitated in re-endothelialized and de-endothelialized aorta by thin-layer chromatography and fluorometric analysis. The results indicate 1) that there was approximately three times as much nonesterified cholesterol and cholesteryl ester in re-endothelialized aorta of Groups I and II as compared with adjacent de-endothelialized aorta and 2) that in re-endothelialized aorta of Group II the amount of total cholesterol correlated with serum cholesterol concentration in contrast to adjacent de-endothelialized aorta, with no correlation over a range of nearly 900 mg/100 ml. These studies indicate that the presence of endothelium favors accumulation of aortic cholesteryl esters. The results suggest that arterial lipid accumulation is not simply a result of passive filtration but may result from metabolic differences in the re-endothelialized neointima.

Alternate JournalAm J Pathol
PubMed ID7361865
PubMed Central IDPMC1903484
Related Faculty: 
David P. Hajjar, Ph.D. Domenick J. Falcone, Ph.D.

Pathology & Laboratory Medicine 1300 York Avenue New York, NY 10065 Phone: (212) 746-6464
Surgical Pathology: (212) 746-2700