|Title||Effects of Escherichia coli lipopolysaccharide on the phosphoinositide metabolism and serotonin secretion in thrombin-activated platelets.|
|Publication Type||Journal Article|
|Year of Publication||1987|
|Authors||Moscat J, Iglesias S, Moreno F, Herrero C, Garcia-Barreno P|
|Keywords||Animals, Blood Platelets, Escherichia coli, In Vitro Techniques, Phosphatidylinositols, Polysaccharides, Bacterial, Serotonin, Swine, Swine, Miniature, Thrombin, Time Factors|
Lipopolysaccharide treatment of platelets in basal conditions does not produce any effect on serotonin secretion or on phosphatidic acid synthesis or arachidonic acid release. However, a brief exposure of platelets to endotoxin enhances the thrombin-stimulated activation of these parameters, whereas a more prolonged treatment with lipopolysaccharide impairs thrombin action. The results presented here also suggest that very short-term treatment of platelets with lipopolysaccharide activates the inositol 1,4,5-trisphosphate 3-kinase. The long-term effects of endotoxin could be mediated by protein kinase C activation.
|Alternate Journal||Circ Shock|
Jorge Moscat, Ph.D.