Title | Distinct Biological Types of Ocular Adnexal Sebaceous Carcinoma: HPV-Driven and Virus-Negative Tumors Arise through Nonoverlapping Molecular-Genetic Alterations. |
Publication Type | Journal Article |
Year of Publication | 2019 |
Authors | Tetzlaff MT, Curry JL, Ning J, Sagiv O, Kandl TL, Peng B, Bell D, Routbort M, Hudgens CW, Ivan D, Kim T-B, Chen K, Eterovic AKarina, Shaw K, Prieto VG, Yemelyanova A, Esmaeli B |
Journal | Clin Cancer Res |
Volume | 25 |
Issue | 4 |
Pagination | 1280-1290 |
Date Published | 2019 02 15 |
ISSN | 1557-3265 |
Keywords | Adult, Aged, Aged, 80 and over, Epidermal Cyst, Eye Neoplasms, Female, Humans, Male, Middle Aged, Mutation, Neoplasms, Adnexal and Skin Appendage, Papillomaviridae, Papillomavirus Infections, Receptors, Notch, Retinoblastoma Binding Proteins, Sequence Analysis, RNA, Tumor Suppressor Protein p53, Ubiquitin-Protein Ligases, Whole Exome Sequencing |
Abstract | PURPOSE: Ocular adnexal (OA) sebaceous carcinoma is an aggressive malignancy of the eyelid and ocular adnexa that frequently recurs and metastasizes, and effective therapies beyond surgical excision are lacking. There remains a critical need to define the molecular-genetic drivers of the disease to understand carcinomagenesis and progression and to devise novel treatment strategies. EXPERIMENTAL DESIGN: We present next-generation sequencing of a targeted panel of cancer-associated genes in 42 and whole transcriptome RNA sequencing from eight OA sebaceous carcinomas from 29 patients. RESULTS: We delineate two potentially distinct molecular-genetic subtypes of OA sebaceous carcinoma. The first is defined by somatic mutations impacting and/or [20/29 (70%) patients, including 10 patients whose primary tumors contained coexisting and mutations] with frequent concomitant mutations affecting genes. These tumors arise in older patients and show frequent local recurrence. The second subtype [9/29 (31%) patients] lacks mutations affecting , or family members, but in 44% (4/9) of these tumors, RNA sequencing and hybridization studies confirm transcriptionally active high-risk human papillomavirus. These tumors arise in younger patients and have not shown local recurrence. CONCLUSIONS: Together, our findings establish a potential molecular-genetic framework by which to understand the development and progression of OA sebaceous carcinoma and provide key molecular-genetic insights to direct the design of novel therapeutic interventions. |
DOI | 10.1158/1078-0432.CCR-18-1688 |
Alternate Journal | Clin Cancer Res |
PubMed ID | 30420449 |
Related Faculty:
Anna Yemelyanova, M.D. Pengbo Zhou, Ph.D.