| Title | Development and evaluation of a novel, semiautomated Clostridium difficile typing platform. |
| Publication Type | Journal Article |
| Year of Publication | 2013 |
| Authors | Westblade LF, Chamberland RR, MacCannell D, Collins R, Dubberke ER, W Dunne M, Burnham C-AD |
| Journal | J Clin Microbiol |
| Volume | 51 |
| Issue | 2 |
| Pagination | 621-4 |
| Date Published | 2013 Feb |
| ISSN | 1098-660X |
| Keywords | Bacterial Typing Techniques, Clostridioides difficile, Cluster Analysis, Electrophoresis, Gel, Pulsed-Field, Reproducibility of Results, Ribotyping, Sensitivity and Specificity |
| Abstract | We describe a novel, semiautomated Clostridium difficile typing platform that is based on PCR-ribotyping in conjunction with a semiautomated molecular typing system. The platform is reproducible with minimal intra- or interassay variability. This method exhibited a discriminatory index of 0.954 and is therefore comparable to more arduous typing systems, such as pulsed-field gel electrophoresis. |
| DOI | 10.1128/JCM.02627-12 |
| Alternate Journal | J Clin Microbiol |
| PubMed ID | 23175261 |
| Grant List | K23AI065806 / AI / NIAID NIH HHS / United States P30 DK52574 / DK / NIDDK NIH HHS / United States |
Related Faculty:
Lars Westblade, Ph.D.