| Title | Detection and characterization of human herpesvirus-8-infected cells in bone marrow biopsies of human immunodeficiency virus-positive patients. |
| Publication Type | Journal Article |
| Year of Publication | 2001 |
| Authors | Meggetto F, Cesarman E, Mourey L, Massip P, Delsol G, Brousset P |
| Journal | Hum Pathol |
| Volume | 32 |
| Issue | 3 |
| Pagination | 288-91 |
| Date Published | 2001 Mar |
| ISSN | 0046-8177 |
| Keywords | Adult, Aged, Biopsy, Bone Marrow, DNA, Viral, Female, Herpesvirus 8, Human, HIV Infections, Humans, Immunohistochemistry, Interleukin-6, Lymphocytes, Male, Middle Aged, Nuclear Proteins, Phosphoproteins, Polymerase Chain Reaction, Sarcoma, Kaposi, Stromal Cells |
| Abstract | We studied 15 bone marrow biopsy specimens from patients with human immunodeficiency virus infection for detection of Kaposi sarcoma herpesvirus (KSHV/HHV-8) DNA sequences by a very sensitive and specific polymerase chain reaction (PCR) assay (with 3 different sets of primers). In addition, we used immunohistochemistry with antiviral interleukin-6 (vIL-6) and anti-latent nuclear antigen-1 (LNA-1) antibodies to localize the infected cells on tissue sections. Among the 15 samples, 6 had positive PCR results with the 3 sets of primers (orf26, orf72, orf75). Interestingly, in 2 of these 6 patients (both with Kaposi sarcoma) vIL-6 and LNA-1 were detected in mononuclear lymphoid cells but not in stromal cells of the bone marrow. The detection of vIL-6--positive lymphoid cells in bone marrow suggests a homing for HHV-8--infected elements in this tissue. The local release of vIL-6 may play some role in the plasmacytosis observed in bone marrow in the acquired immunodeficiency syndrome. HUM PATHOL 32:288-291. |
| DOI | 10.1053/hupa.2001.22749 |
| Alternate Journal | Hum Pathol |
| PubMed ID | 11274637 |
| Grant List | CA68939 / CA / NCI NIH HHS / United States |
Related Faculty:
Ethel Cesarman, M.D., Ph.D.