Title | Crystal structure of the in vivo-assembled Bacillus subtilis Spx/RNA polymerase alpha subunit C-terminal domain complex. |
Publication Type | Journal Article |
Year of Publication | 2009 |
Authors | Lamour V, Westblade LF, Campbell EA, Darst SA |
Journal | J Struct Biol |
Volume | 168 |
Issue | 2 |
Pagination | 352-6 |
Date Published | 2009 Nov |
ISSN | 1095-8657 |
Keywords | Bacillus subtilis, Crystallography, X-Ray, DNA-Directed RNA Polymerases, Protein Structure, Secondary |
Abstract | The Bacillus subtilis Spx protein is a global transcription factor that interacts with the C-terminal domain of the RNA polymerase alpha subunit (alphaCTD) and regulates transcription of genes involved in thiol-oxidative stress, sporulation, competence, and organosulfur metabolism. Here we determined the X-ray crystal structure of the Spx/alphaCTD complex from an entirely new crystal form than previously reported [Newberry, K.J., Nakano, S., Zuber, P., Brennan, R.G., 2005. Crystal structure of the Bacillus subtilis anti-alpha, global transcriptional regulator, Spx, in complex with the alpha C-terminal domain of RNA polymerase. Proc. Natl. Acad. Sci. USA 102, 15839-15844]. Comparison of the previously reported sulfate-bound complex and our sulfate-free complex reveals subtle conformational changes that may be important for the role of Spx in regulating organosulfur metabolism. |
DOI | 10.1016/j.jsb.2009.07.001 |
Alternate Journal | J Struct Biol |
PubMed ID | 19580872 |
Grant List | GM063759 / GM / NIGMS NIH HHS / United States |
Related Faculty:
Lars Westblade, Ph.D.