Title | Biosynthesis of plant-specific flavones and flavonols in Streptomyces venezuelae. |
Publication Type | Journal Article |
Year of Publication | 2010 |
Authors | Park SRyeol, Paik JHye, Ahn MSun, Park JWon, Yoon YJoon |
Journal | J Microbiol Biotechnol |
Volume | 20 |
Issue | 9 |
Pagination | 1295-9 |
Date Published | 2010 Sep |
ISSN | 1017-7825 |
Keywords | Apiaceae, Citrus, Flavones, Flavonols, Gene Expression, Genes, Plant, Genetic Engineering, Industrial Microbiology, Mixed Function Oxygenases, Streptomyces |
Abstract | Recently, recombinant Streptomyces venezuelae has been established as a heterologous host for microbial production of flavanones and stilbenes, a class of plant-specific polyketides. In the present work, we expanded the applicability of the S. venezuelae system to the production of more diverse plant polyketides including flavones and flavonols. A plasmid with the synthetic codon-optimized flavone synthase I gene from Petroselium crispum was introduced to S. venezuelae DHS2001 bearing a deletion of the native pikromycin polyketide synthase gene, and the resulting strain generated flavones from exogenously fed flavanones. In addition, a recombinant S. venezuelae mutant expressing a codon-optimized flavanone 3beta-hydroxylase gene from Citrus siensis and a flavonol synthase gene from Citrus unshius also successfully produced flavonols. |
DOI | 10.4014/jmb.1005.05038 |
Alternate Journal | J Microbiol Biotechnol |
PubMed ID | 20890094 |
Related Faculty:
Ji-Hye Paik, Ph.D.