Adenovirus E1A and E1B genes are regulated posttranscriptionally in human lymphoid cells.

TitleAdenovirus E1A and E1B genes are regulated posttranscriptionally in human lymphoid cells.
Publication TypeJournal Article
Year of Publication1990
AuthorsLavery DJ, Chen-Kiang S
JournalJ Virol
Date Published1990 Nov
KeywordsAdenovirus Early Proteins, Adenoviruses, Human, Blotting, Southern, Gene Expression Regulation, Viral, HeLa Cells, Humans, In Vitro Techniques, Lymphocytes, Oncogene Proteins, Viral, Plasmacytoma, Precipitin Tests, RNA Processing, Post-Transcriptional, RNA, Messenger, RNA, Viral, Transcription, Genetic, Tumor Cells, Cultured

The interactions of adenovirus with differentiated human cells have been investigated in human myeloma cells. Relative to HeLa cells, the E1A and E1B genes, but not other viral genes, were markedly repressed by differential RNA stabilization, resulting in 20- to 50-fold less E1A and E1B mRNAs at steady state late in infection. The reduced E1A level corresponded to an approximately 200-fold-lower abundance of E1A polypeptides, which were nonetheless capable of efficient transactivation of E1A-dependent viral genes and were necessary for productive infection. The E1B gene was further regulated posttranscriptionally, yielding altered molar representation of alternatively spliced 22S and 13S mRNAs early in infection of myeloma cells. Taken together, these results suggested that repression and altered expression of E1A and E1B genes may provide a molecular basis of delayed kinetics of infection of lymphoid cells with adenovirus (D. Lavery, S. M. Fu, T. Lufkin, and S. Chen-Kiang, J. Virol. 61:1466-1472, 1987). The molecular mechanisms by which E1A and E1B are regulated and by which E1A transactivates viral genes in lymphoid cells are discussed.

Alternate JournalJ Virol
PubMed ID2145444
PubMed Central IDPMC248584
Grant ListAI 24404 / AI / NIAID NIH HHS / United States
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Selina Chen-Kiang, Ph.D.

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