Title | Accumulation of miR-155 and BIC RNA in human B cell lymphomas. |
Publication Type | Journal Article |
Year of Publication | 2005 |
Authors | Eis PS, Tam W, Sun L, Chadburn A, Li Z, Gomez MF, Lund E, Dahlberg JE |
Journal | Proc Natl Acad Sci U S A |
Volume | 102 |
Issue | 10 |
Pagination | 3627-32 |
Date Published | 2005 Mar 08 |
ISSN | 0027-8424 |
Keywords | Cell Line, Tumor, Humans, Lymphoma, B-Cell, Lymphoma, Large B-Cell, Diffuse, MicroRNAs, RNA, Messenger, RNA, Untranslated |
Abstract | We show that the microRNA miR-155 can be processed from sequences present in BIC RNA, a spliced and polyadenylated but non-protein-coding RNA that accumulates in lymphoma cells. The precursor of miR-155 is likely a transient spliced or unspliced nuclear BIC transcript rather than accumulated BIC RNA, which is primarily cytoplasmic. By using a sensitive and quantitative assay, we find that clinical isolates of several types of B cell lymphomas, including diffuse large B cell lymphoma (DLBCL), have 10- to 30-fold higher copy numbers of miR-155 than do normal circulating B cells. Similarly, the quantities of BIC RNA are elevated in lymphoma cells, but ratios of the amounts of the two RNAs are not constant, suggesting that the level of miR-155 is controlled by transcription and processing. Significantly higher levels of miR-155 are present in DLBCLs with an activated B cell phenotype than with the germinal center phenotype. Because patients with activated B cell-type DLBCL have a poorer clinical prognosis, quantification of this microRNA may be diagnostically useful. |
DOI | 10.1073/pnas.0500613102 |
Alternate Journal | Proc Natl Acad Sci U S A |
PubMed ID | 15738415 |
PubMed Central ID | PMC552785 |
Grant List | R37 GM030220 / GM / NIGMS NIH HHS / United States GM 30220 / GM / NIGMS NIH HHS / United States |
Related Faculty:
Amy Chadburn, M.D.