Title | Epithelial aPKC deficiency leads to stem cell loss preceding metaplasia in colorectal cancer initiation. |
Publication Type | Journal Article |
Year of Publication | 2024 |
Authors | Kinoshita H, Martinez-Ordoñez A, Cid-Diaz T, Han Q, Duran A, Muta Y, Zhang X, Linares JF, Nakanishi Y, Kasashima H, Yashiro M, Maeda K, Albaladejo-Gonzalez A, Torres-Moreno D, García-Solano J, Conesa-Zamora P, Inghirami G, Diaz-Meco MT, Moscat J |
Journal | Dev Cell |
Volume | 59 |
Issue | 15 |
Pagination | 1972-1987.e8 |
Date Published | 2024 Aug 05 |
ISSN | 1878-1551 |
Keywords | Adaptor Proteins, Signal Transducing, Animals, Cell Lineage, Cell Transformation, Neoplastic, Colorectal Neoplasms, Epithelial Cells, Humans, Intestinal Mucosa, Isoenzymes, Metaplasia, Mice, Neoplastic Stem Cells, Organoids, Protein Kinase C, Stem Cells, Transcription Factor AP-1, YAP-Signaling Proteins |
Abstract | The early mechanisms of spontaneous tumor initiation that precede malignancy are largely unknown. We show that reduced aPKC levels correlate with stem cell loss and the induction of revival and metaplastic programs in serrated- and conventional-initiated premalignant lesions, which is perpetuated in colorectal cancers (CRCs). Acute inactivation of PKCλ/ι in vivo and in mouse organoids is sufficient to stimulate JNK in non-transformed intestinal epithelial cells (IECs), which promotes cell death and the rapid loss of the intestinal stem cells (ISCs), including those that are LGR5+. This is followed by the accumulation of revival stem cells (RSCs) at the bottom of the crypt and fetal-metaplastic cells (FMCs) at the top, creating two spatiotemporally distinct cell populations that depend on JNK-induced AP-1 and YAP. These cell lineage changes are maintained during cancer initiation and progression and determine the aggressive phenotype of human CRC, irrespective of their serrated or conventional origin. |
DOI | 10.1016/j.devcel.2024.05.001 |
Alternate Journal | Dev Cell |
PubMed ID | 38815584 |
PubMed Central ID | PMC11303105 |
Grant List | R50 CA265332 / CA / NCI NIH HHS / United States R01 CA250025 / CA / NCI NIH HHS / United States R50 CA283476 / CA / NCI NIH HHS / United States R01 CA246765 / CA / NCI NIH HHS / United States R01 CA275846 / CA / NCI NIH HHS / United States R01 CA265892 / CA / NCI NIH HHS / United States |
Related Faculty:
Maria Angeles Duran, Ph.D. Dr. Xiao Zhang Giorgio Inghirami, M.D. Maria Diaz-Meco Conde, Ph.D. Jorge Moscat, Ph.D.