Title | Detecting high-grade cervical disease on ASC-H cytology: role of BD ProEx C and Digene Hybrid Capture II HPV DNA testing. |
Publication Type | Journal Article |
Year of Publication | 2008 |
Authors | Siddiqui MT, Cohen C, Nassar A |
Journal | Am J Clin Pathol |
Volume | 130 |
Issue | 5 |
Pagination | 765-70 |
Date Published | 2008 Nov |
ISSN | 1943-7722 |
Keywords | Adult, Aged, Antigens, Neoplasm, Cell Cycle Proteins, Cervical Intraepithelial Neoplasia, DNA Topoisomerases, Type II, DNA, Viral, DNA-Binding Proteins, Female, Humans, Middle Aged, Minichromosome Maintenance Complex Component 2, Nuclear Proteins, Papillomaviridae, Papillomavirus Infections, Precancerous Conditions, Reagent Kits, Diagnostic, Sensitivity and Specificity, Tumor Virus Infections, Uterine Cervical Neoplasms |
Abstract | The BD ProEx C stain (BD Diagnostics-Tripath, Burlington, NC) detects 2 molecular markers known to be overexpressed in cervical squamous cell carcinoma, minichromosome maintenance protein 2 and topoisomerase II. This study was conducted to evaluate this stain detecting high-grade cervical disease, and results were compared with high-risk HPV (hr-HPV) status and histologic biopsy diagnosis in Papanicolaou tests with atypical squamous cells, cannot exclude high-grade squamous intraepithelial lesion (ASC-H) cytologic results. Cervical cytology specimens were collected in BD SurePath preservative (BD Diagnostics-Tripath), and 100 specimens with a diagnosis of ASC-H were included in the study. A second BD SurePath slide, prepared from the residual cellular material from these ASC-H cases, was immunostained using the BD ProEx C reagent. Nuclear staining of ASC-H cells was considered a positive result. The sensitivity for BD ProEx C for detecting cervical intraepithelial neoplasia 2 or worse was 98.8% and for hr-HPV was 71.1%, whereas specificity values were 82.4% and 64.7%, respectively. BD ProEx C has potential as a marker for detection of high-grade disease in patients with ASC-H cytologic results. |
DOI | 10.1309/AJCPWW6V2KGXODUI |
Alternate Journal | Am J Clin Pathol |
PubMed ID | 18854269 |
Related Faculty:
Momin Siddiqui, M.D.