Multicenter validation of the VITEK MS v2.0 MALDI-TOF mass spectrometry system for the identification of fastidious gram-negative bacteria.

TitleMulticenter validation of the VITEK MS v2.0 MALDI-TOF mass spectrometry system for the identification of fastidious gram-negative bacteria.
Publication TypeJournal Article
Year of Publication2014
AuthorsBranda JA, Rychert J, Burnham C-AD, Bythrow M, Garner OB, Ginocchio CC, Jennemann R, Lewinski MA, Manji R, A Mochon B, Procop GW, Richter SS, Sercia LF, Westblade LF, Ferraro MJane
JournalDiagn Microbiol Infect Dis
Volume78
Issue2
Pagination129-31
Date Published2014 Feb
ISSN1879-0070
KeywordsBacterial Typing Techniques, Gram-Negative Bacteria, Reference Standards, Reproducibility of Results, RNA, Bacterial, RNA, Ribosomal, 16S, Sensitivity and Specificity, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Abstract

The VITEK MS v2.0 MALDI-TOF mass spectrometry system's performance in identifying fastidious gram-negative bacteria was evaluated in a multicenter study. Compared with the reference method (DNA sequencing), the VITEK MS system provided an accurate, species-level identification for 96% of 226 isolates; an additional 1% were accurately identified to the genus level.

DOI10.1016/j.diagmicrobio.2013.08.013
Alternate JournalDiagn Microbiol Infect Dis
PubMed ID24321357
Related Faculty: 
Lars Westblade, Ph.D.

Pathology & Laboratory Medicine 1300 York Avenue New York, NY 10065 Phone: (212) 746-6464
Surgical Pathology: (212) 746-2700