| Title | Genomic and proteomic analysis of phiEco32, a novel Escherichia coli bacteriophage. |
| Publication Type | Journal Article |
| Year of Publication | 2008 |
| Authors | Savalia D, Westblade LF, Goel M, Florens L, Kemp P, Akulenko N, Pavlova O, Padovan JC, Chait BT, Washburn MP, Ackermann H-W, Mushegian A, Gabisonia T, Molineux I, Severinov K |
| Journal | J Mol Biol |
| Volume | 377 |
| Issue | 3 |
| Pagination | 774-89 |
| Date Published | 2008 Mar 28 |
| ISSN | 1089-8638 |
| Keywords | Coliphages, DNA-Directed RNA Polymerases, Gene Expression Regulation, Bacterial, Gene Expression Regulation, Viral, Genome, Viral, Genomics, Promoter Regions, Genetic, Proteome, Proteomics |
| Abstract | A novel bacteriophage infecting Escherichia coli was isolated during a large-scale screen for bacteriophages that may be used for therapy of mastitis in cattle. The 77,554-bp genome of the bacteriophage, named phiEco32, was sequenced and annotated, and its virions were characterized by electron microscopy and proteomics. Two phiEco32-encoded proteins that interact with host RNA polymerase were identified. One of them is an ECF family sigma factor that may be responsible for transcription of some viral genes. Another RNA polymerase-binding protein is a novel transcription inhibitor whose mechanism of action remains to be defined. |
| DOI | 10.1016/j.jmb.2007.12.077 |
| Alternate Journal | J Mol Biol |
| PubMed ID | 18294652 |
| Grant List | R01 GM32095 / GM / NIGMS NIH HHS / United States RR022220 / RR / NCRR NIH HHS / United States T90DK070135 / DK / NIDDK NIH HHS / United States R01 GM59295 / GM / NIGMS NIH HHS / United States GM61898 / GM / NIGMS NIH HHS / United States RR00862 / RR / NCRR NIH HHS / United States |
Related Faculty:
Lars Westblade, Ph.D.