| Title | The Escherichia coli regulator of sigma 70 protein, Rsd, can up-regulate some stress-dependent promoters by sequestering sigma 70. |
| Publication Type | Journal Article |
| Year of Publication | 2007 |
| Authors | Mitchell JE, Oshima T, Piper SE, Webster CL, Westblade LF, Karimova G, Ladant D, Kolb A, Hobman JL, Busby SJW, Lee DJ |
| Journal | J Bacteriol |
| Volume | 189 |
| Issue | 9 |
| Pagination | 3489-95 |
| Date Published | 2007 May |
| ISSN | 0021-9193 |
| Keywords | Amino Acid Substitution, Artificial Gene Fusion, Bacterial Proteins, beta-Galactosidase, DNA-Directed RNA Polymerases, Escherichia coli, Escherichia coli Proteins, Gene Expression Profiling, Gene Expression Regulation, Bacterial, Genes, Reporter, Lac Operon, Mutation, Missense, Oligonucleotide Array Sequence Analysis, Promoter Regions, Genetic, Protein Binding, Repressor Proteins, Sigma Factor, Two-Hybrid System Techniques, Up-Regulation |
| Abstract | The Escherichia coli Rsd protein forms complexes with the RNA polymerase sigma(70) factor, but its biological role is not understood. Transcriptome analysis shows that overexpression of Rsd causes increased expression from some promoters whose expression depends on the alternative sigma(38) factor, and this was confirmed by experiments with lac fusions at selected promoters. The LP18 substitution in Rsd increases the Rsd-dependent stimulation of these promoter-lac fusions. Analysis with a bacterial two-hybrid system shows that the LP18 substitution in Rsd increases its interaction with sigma(70). Our experiments support a model in which the role of Rsd is primarily to sequester sigma(70), thereby increasing the levels of RNA polymerase containing the alternative sigma(38) factor. |
| DOI | 10.1128/JB.00019-07 |
| Alternate Journal | J Bacteriol |
| PubMed ID | 17351046 |
| PubMed Central ID | PMC1855875 |
| Grant List | / / Wellcome Trust / United Kingdom |
Related Faculty:
Lars Westblade, Ph.D.