Title | An Orthotopic Implantation Mouse Model of Hepatocellular Carcinoma with Underlying Liver Steatosis. |
Publication Type | Journal Article |
Year of Publication | 2020 |
Authors | Kasashima H, Duran A, Cid-Diaz T, Kudo Y, Diaz-Meco MT, Moscat J |
Journal | STAR Protoc |
Volume | 1 |
Issue | 3 |
Pagination | 100185 |
Date Published | 2020 Dec 18 |
ISSN | 2666-1667 |
Abstract | This protocol provides the steps required for a mouse liver orthotopic implantation model. The reliable pre-clinical animal models that have similar characteristics to hepatocellular carcinoma (HCC) are a powerful tool to unveil the mechanisms controlling tumor initiation and progression. Here, we describe a syngeneic orthotopic HCC model that recapitulates the role of a host pro-tumorigenic microenvironment by pre-conditioning mouse livers with a high-fat diet (HFD). For complete details on the use and execution of this protocol, please refer to Kudo et al. (2020). |
DOI | 10.1016/j.xpro.2020.100185 |
Alternate Journal | STAR Protoc |
PubMed ID | 33377079 |
PubMed Central ID | PMC7757567 |
Grant List | P30 CA030199 / CA / NCI NIH HHS / United States R01 CA211794 / CA / NCI NIH HHS / United States R01 DK108743 / DK / NIDDK NIH HHS / United States |
Related Faculty:
Jorge Moscat, Ph.D. Maria Diaz-Meco Conde, Ph.D.