| Title | LNCIB human full-length cDNAs collection: towards a better comprehension of the human transcriptome. |
| Publication Type | Journal Article |
| Year of Publication | 2003 |
| Authors | Dalla E, Verardo R, Lazarević D, Marchionni L, Reid JF, Bahar N, Klaric E, Marcuzzi G, Marzio R, Belgrano A, Licastro D, Schneider C |
| Journal | C R Biol |
| Volume | 326 |
| Issue | 10-11 |
| Pagination | 967-70 |
| Date Published | 2003 Oct-Nov |
| ISSN | 1631-0691 |
| Keywords | Databases, Factual, Gene Library, Genome, Human, Humans, Transcription, Genetic |
| Abstract | LNCIB has been producing a variety of human full-length-enriched, normalized and subtracted cDNA libraries from various cell lines and tissues in different developmental stages by using the CAP-Trapper method. By sequencing 23000 clones of these libraries we identified a pool of about 5800 good quality unique cDNAs. After BLAST analysis on Human RefSeq/Unigene databases, 1717 of these sequences remained with no or poor annotation. We show that cross-species comparative BLAST resulted as a valid tool for the annotation of orthologous genes. |
| DOI | 10.1016/j.crvi.2003.09.029 |
| Alternate Journal | C R Biol |
| PubMed ID | 14744102 |
| Grant List | UO1CA 80200 / CA / NCI NIH HHS / United States |
Related Faculty:
Luigi Marchionni, M.D., Ph.D.