Title | Detection of Fusions: Merits and Limitations of Current Diagnostic Platforms. |
Publication Type | Journal Article |
Year of Publication | 2019 |
Authors | Solomon JP, Hechtman JF |
Journal | Cancer Res |
Volume | 79 |
Issue | 13 |
Pagination | 3163-3168 |
Date Published | 2019 07 01 |
ISSN | 1538-7445 |
Keywords | Biomarkers, Tumor, Gene Rearrangement, Humans, Neoplasms, Oncogene Proteins, Fusion, Receptor, trkA, Receptors, Nerve Growth Factor |
Abstract | Oncogenic fusions involving , and with various partners are diagnostic of infantile fibrosarcoma and secretory carcinoma yet also occur in lower frequencies across many types of malignancies. Recently, targeted small molecular inhibitor therapy has been shown to induce a durable response in a high percentage of patients with fusion-positive cancers, which has made the detection of fusions critical. Several techniques for fusion diagnosis exist, including pan-Trk IHC, FISH, reverse transcription PCR, DNA-based next-generation sequencing (NGS), and RNA-based NGS. Each of these assays has unique features, advantages, and limitations, and familiarity with these assays is critical to appropriately screen for fusions. Here, we review the details of each existing methodology. |
DOI | 10.1158/0008-5472.CAN-19-0372 |
Alternate Journal | Cancer Res |
PubMed ID | 31196931 |
PubMed Central ID | PMC6606326 |
Grant List | P30 CA008748 / CA / NCI NIH HHS / United States |
Related Faculty:
James Solomon, M.D., Ph.D.