| Title | Yph1p, an ORC-interacting protein: potential links between cell proliferation control, DNA replication, and ribosome biogenesis. |
| Publication Type | Journal Article |
| Year of Publication | 2002 |
| Authors | Du Y-CNancy, Stillman B |
| Journal | Cell |
| Volume | 109 |
| Issue | 7 |
| Pagination | 835-48 |
| Date Published | 2002 Jun 28 |
| ISSN | 0092-8674 |
| Keywords | Blotting, Western, Cell Division, Cell Nucleolus, DNA Replication, DNA, Fungal, DNA-Binding Proteins, Flow Cytometry, Histone Deacetylases, Mass Spectrometry, Mutation, Nuclear Proteins, Origin Recognition Complex, Protein Binding, Protein Subunits, Resting Phase, Cell Cycle, Ribosomes, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Sequence Homology, Amino Acid, Silent Information Regulator Proteins, Saccharomyces cerevisiae, Sirtuin 2, Sirtuins, Trans-Activators |
| Abstract | Immunoprecipitation of the origin recognition complex (ORC) from yeast extracts identified Yph1p, an essential protein containing a BRCT domain. Two Yph1p complexes were characterized. Besides ORC, MCM proteins, cell-cycle regulatory proteins, checkpoint proteins, 60S ribosomal proteins, and preribosome particle proteins were found to be associated with Yph1p. Yph1p is predominantly nucleolar and is required for 60S ribosomal subunit biogenesis and possibly for translation on polysomes. Proliferating cells depleted of Yph1p arrest in G(1) or G(2), with no cells in S phase, or significantly delay S phase progression after release from a hydroxyurea arrest. Yph1p levels decline as cells commit to exit the cell cycle, and levels vary depending on energy source. Yph1p may link cell proliferation control to DNA replication, ribosome biogenesis, and translation on polysomes. |
| DOI | 10.1016/s0092-8674(02)00773-0 |
| Alternate Journal | Cell |
| PubMed ID | 12110181 |
| Grant List | R01 GM045436 / GM / NIGMS NIH HHS / United States GM45436 / GM / NIGMS NIH HHS / United States |
Related Lab:
Related Faculty:
Yi-Chieh (Nancy) Du, Ph.D.