Type I interferon and neutrophil transcripts in lupus nephritis renal biopsies: clinical and histopathological associations.

TitleType I interferon and neutrophil transcripts in lupus nephritis renal biopsies: clinical and histopathological associations.
Publication TypeJournal Article
Year of Publication2023
AuthorsMavragani CP, Kirou KA, Seshan SV, Crow MK
JournalRheumatology (Oxford)
Date Published2023 Jul 05
KeywordsBiopsy, Defensins, Humans, Kidney, Lupus Nephritis, Neutrophils

OBJECTIVES: To investigate the expression of type I IFN (IFN-I) and neutrophil transcripts in kidney tissue from patients with different classes of LN and their association with distinct clinical and histopathological features.

METHODS: Quantitation of IFN-I, defensin-α3 and formyl peptide receptor-like 1 (FPRL-1) transcripts was performed in kidney biopsy tissue from 24 patients with various classes of LN (6 class III, 14 class IV, 4 class V) and 3 control samples. Patient demographics, glomerular filtration rate (eGFR) and histopathological characteristics, including activity and chronicity indices, were analysed.

RESULTS: IFNα2 and IFNβ transcripts were overexpressed in renal tissues from patients with proliferative forms of LN (III/IV) compared with patients with membranous nephritis and control kidneys. Patients with LN and impaired renal function, attested by eGFR, displayed higher relative expression of IFNα2 transcripts in renal tissues compared with those with normal renal function (23.0 ± 16.2 vs 12.0 ± 14.8, P = 0.04). Defensin-α3, but not FPRL-1, transcripts were overexpressed in LN tissues, particularly those with segmental necrotizing lesions, and were correlated with higher renal pathological activity indices (r = 0.61, P = 0.02), urinary protein levels (r = 0.44, P = 0.048) and IFNα2 expression (r = 0.50, P = 0.01).

CONCLUSION: IFN-I transcripts are expressed locally in kidneys from patients with proliferative LN and are associated with impaired renal function. Elevated defensin-α3 transcripts, a neutrophil product associated with neutrophil extracellular traps, may identify a driver of local IFN-I expression. These findings provide insights into the mechanisms of proliferative LN and may inform therapeutic decisions regarding selection of IFN-I pathway inhibitors.

Alternate JournalRheumatology (Oxford)
PubMed ID36355567
PubMed Central IDPMC10321084
Grant ListR01 AI059893 / AI / NIAID NIH HHS / United States
Related Faculty: 
Surya V. Seshan, M.D.

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