Transformation and plasmacytoid differentiation of EBV-infected human B lymphoblasts by ras oncogenes.

TitleTransformation and plasmacytoid differentiation of EBV-infected human B lymphoblasts by ras oncogenes.
Publication TypeJournal Article
Year of Publication1989
AuthorsSeremetis S, Inghirami G, Ferrero D, Newcomb EW, Knowles DM, Dotto GP, Dalla-Favera R
Date Published1989 Feb 03
KeywordsAnimals, B-Lymphocytes, Cell Differentiation, Cell Transformation, Neoplastic, Cell Transformation, Viral, DNA Replication, Flow Cytometry, Fluorescent Antibody Technique, Gene Expression Regulation, Genes, ras, Herpesvirus 4, Human, Humans, Mice, Mice, Nude, Neoplasm Transplantation, Neoplasms, Experimental, Phenotype, Plasma Cells

The biological effects of ras oncogene activation in B cells were studied by using amphotropic retroviral vectors to introduce H- or N-ras oncogenes into human B lymphoblasts immortalized by Epstein-Barr virus. Expression of both H- and N-ras oncogenes led to malignant transformation of these cells, as shown by clonogenicity in semisolid media and tumorigenicity in immunodeficient mice. In addition, terminal differentiation into plasma cells was detectable as specific changes in morphology, immunoglobulin secretion, and cell surface antigen expression. This combined effect, promoting growth and differentiation in human lymphoblasts, represents a novel biological action of ras oncogenes and has implications for the pathogenesis of terminally differentiated B-lymphoid malignancies such as multiple myeloma.

Alternate JournalScience
PubMed ID2536954
Grant ListCA-37165 / CA / NCI NIH HHS / United States
CA49236 / CA / NCI NIH HHS / United States
EY 06337 / EY / NEI NIH HHS / United States
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Giorgio Inghirami, M.D.

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