|Title||Subendothelial deposition of von Willebrand's factor requires the presence of endothelial cells.|
|Publication Type||Journal Article|
|Year of Publication||1982|
|Authors||Sussman II, Rand JH|
|Journal||J Lab Clin Med|
|Date Published||1982 Oct|
|Keywords||Animals, Aorta, Aorta, Abdominal, Aorta, Thoracic, Blood Coagulation Factors, Endothelium, Fluorescent Antibody Technique, Male, Microscopy, Fluorescence, Rabbits, von Willebrand Factor|
The presence of FVIII/VWF in human aortic subendothelium has been previously established. The present study was undertaken to determine the origin of this FVIII/VWF. Indirect immunofluorescence microscopy was employed to detect FVIII/VWF in rabbit aortas, after passage of a balloon catheter. Immediately after this de-endothelialization, FVIII/VWF was found to be present directly luminal to the internal elastic lamina. However, in the subsequent 5 days to 2 months, no FVIII/VWF was found on the luminal surface of exposed, nonre-endothelialized neointima. After endothelial cell regrowth, however, FVIII/VWF was again seen on the luminal surface. Thus these studies reveal that the deposition of subendothelial FVIII/VWF in neointima requires the presence of endothelial cells and that relatively trivial amounts of plasmatic FVIII/VWF are deposited on neointimal connective tissue after de-endothelialization.
|Alternate Journal||J Lab Clin Med|
|Grant List||HL 16387 / HL / NHLBI NIH HHS / United States|
Jacob H. Rand, M.D.