Subendothelial deposition of von Willebrand's factor requires the presence of endothelial cells.

TitleSubendothelial deposition of von Willebrand's factor requires the presence of endothelial cells.
Publication TypeJournal Article
Year of Publication1982
AuthorsSussman II, Rand JH
JournalJ Lab Clin Med
Date Published1982 Oct
KeywordsAnimals, Aorta, Aorta, Abdominal, Aorta, Thoracic, Blood Coagulation Factors, Endothelium, Fluorescent Antibody Technique, Male, Microscopy, Fluorescence, Rabbits, von Willebrand Factor

The presence of FVIII/VWF in human aortic subendothelium has been previously established. The present study was undertaken to determine the origin of this FVIII/VWF. Indirect immunofluorescence microscopy was employed to detect FVIII/VWF in rabbit aortas, after passage of a balloon catheter. Immediately after this de-endothelialization, FVIII/VWF was found to be present directly luminal to the internal elastic lamina. However, in the subsequent 5 days to 2 months, no FVIII/VWF was found on the luminal surface of exposed, nonre-endothelialized neointima. After endothelial cell regrowth, however, FVIII/VWF was again seen on the luminal surface. Thus these studies reveal that the deposition of subendothelial FVIII/VWF in neointima requires the presence of endothelial cells and that relatively trivial amounts of plasmatic FVIII/VWF are deposited on neointimal connective tissue after de-endothelialization.

Alternate JournalJ Lab Clin Med
PubMed ID6750017
Grant ListHL 16387 / HL / NHLBI NIH HHS / United States
Related Faculty: 
Jacob H. Rand, M.D.

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