Title | Size-selective opening of the blood-brain barrier by targeting endothelial sphingosine 1-phosphate receptor 1. |
Publication Type | Journal Article |
Year of Publication | 2017 |
Authors | Yanagida K, Liu CH, Faraco G, Galvani S, Smith HK, Burg N, Anrather J, Sanchez T, Iadecola C, Hla T |
Journal | Proc Natl Acad Sci U S A |
Volume | 114 |
Issue | 17 |
Pagination | 4531-4536 |
Date Published | 2017 04 25 |
ISSN | 1091-6490 |
Keywords | Animals, Biological Transport, Blood-Brain Barrier, Brain, Endothelial Cells, Endothelium, Vascular, Gene Expression Regulation, Lysophospholipids, Mice, Mice, Knockout, Receptors, Lysosphingolipid, Sphingosine, Tight Junctions |
Abstract | The vasculature of the central nervous system (CNS) forms a selective barrier termed the blood-brain barrier (BBB). Disruption of the BBB may contribute to various CNS diseases. Conversely, the intact BBB restricts efficient penetration of CNS-targeted drugs. Here, we report the BBB-regulatory role of endothelial sphingosine 1-phosphate (S1P) receptor-1, a G protein-coupled receptor known to promote the barrier function in peripheral vessels. Endothelial-specific knockout mice ( ) showed BBB breach for small-molecular-mass fluorescence tracers (<3 kDa), but not larger tracers (>10 kDa). Chronic BBB leakiness was associated with cognitive impairment, as assessed by the novel object recognition test, but not signs of brain inflammation. Brain microvessels of mice showed altered subcellular distribution of tight junctional proteins. Pharmacological inhibition of S1P function led to transient BBB breach. These data suggest that brain endothelial S1P maintain the BBB by regulating the proper localization of tight junction proteins and raise the possibility that endothelial S1P inhibition may be a strategy for transient BBB opening and delivery of small molecules into the CNS. |
DOI | 10.1073/pnas.1618659114 |
Alternate Journal | Proc Natl Acad Sci U S A |
PubMed ID | 28396408 |
PubMed Central ID | PMC5410849 |
Grant List | R01 NS037853 / NS / NINDS NIH HHS / United States R01 NS100447 / NS / NINDS NIH HHS / United States U54 HL117798 / HL / NHLBI NIH HHS / United States R01 NS095441 / NS / NINDS NIH HHS / United States R01 HL094465 / HL / NHLBI NIH HHS / United States R37 NS089323 / NS / NINDS NIH HHS / United States R01 HL089934 / HL / NHLBI NIH HHS / United States R35 HL135821 / HL / NHLBI NIH HHS / United States |
Related Faculty:
Teresa Sanchez, Ph.D.