| Title | Role of prostaglandin H synthase isoforms in murine ear edema induced by phorbol ester application on skin. |
| Publication Type | Journal Article |
| Year of Publication | 1999 |
| Authors | Sanchez T, Moreno JJ |
| Journal | Prostaglandins Other Lipid Mediat |
| Volume | 57 |
| Issue | 2-3 |
| Pagination | 119-31 |
| Date Published | 1999 May |
| ISSN | 1098-8823 |
| Keywords | Animals, Anti-Inflammatory Agents, Anti-Inflammatory Agents, Non-Steroidal, Arachidonic Acid, Cyclooxygenase 1, Cyclooxygenase 2, Dexamethasone, Ear Diseases, Edema, Eicosanoids, Inflammation, Isoenzymes, Male, Membrane Proteins, Mice, Prostaglandin-Endoperoxide Synthases, Prostaglandins, Skin, Tetradecanoylphorbol Acetate, Time Factors |
| Abstract | Topical application of TPA to a murine ear induced an edema that was accompanied by eicosanoid biosynthesis and an early enhancement of prostaglandin H synthase 2 (PGHS-2) expression. PGHS-2 induction may be correlated with the time-course of TPA-induced edema formation. Treatment with drugs that inhibit AA mobilization such as dexamethasone or manoalide or inhibitors of leukotriene formation such as zileuton or baicalein, reduced TPA-induced edema development and PGHS-2 levels. On the other hand, arachidonic acid (AA) application on the murine ear induced rapid expression of PGHS-2. This effect was not reproduced by other fatty acids such as oleic, linoleic, eicosatetraynoic or eicosapentaenoic acids. PGHS-2 expression induced by AA application was independent of PGHS and lipoxygenase metabolite synthesis. However, topical application of PGE2 on skin induced PGHS-2 overexpression. This study suggests that AA release and/or subsequent metabolism by PGHS may be involved in the induction of PGHS-2 expression in murine TPA- and AA-induced ear oedema. |
| DOI | 10.1016/s0090-6980(98)00078-1 |
| Alternate Journal | Prostaglandins Other Lipid Mediat |
| PubMed ID | 10410383 |
Related Faculty:
Teresa Sanchez, Ph.D.