Role of prostaglandin H synthase isoforms in murine ear edema induced by phorbol ester application on skin.

TitleRole of prostaglandin H synthase isoforms in murine ear edema induced by phorbol ester application on skin.
Publication TypeJournal Article
Year of Publication1999
AuthorsSanchez T, Moreno JJ
JournalProstaglandins Other Lipid Mediat
Date Published1999 May
KeywordsAnimals, Anti-Inflammatory Agents, Anti-Inflammatory Agents, Non-Steroidal, Arachidonic Acid, Cyclooxygenase 1, Cyclooxygenase 2, Dexamethasone, Ear Diseases, Edema, Eicosanoids, Inflammation, Isoenzymes, Male, Membrane Proteins, Mice, Prostaglandin-Endoperoxide Synthases, Prostaglandins, Skin, Tetradecanoylphorbol Acetate, Time Factors

Topical application of TPA to a murine ear induced an edema that was accompanied by eicosanoid biosynthesis and an early enhancement of prostaglandin H synthase 2 (PGHS-2) expression. PGHS-2 induction may be correlated with the time-course of TPA-induced edema formation. Treatment with drugs that inhibit AA mobilization such as dexamethasone or manoalide or inhibitors of leukotriene formation such as zileuton or baicalein, reduced TPA-induced edema development and PGHS-2 levels. On the other hand, arachidonic acid (AA) application on the murine ear induced rapid expression of PGHS-2. This effect was not reproduced by other fatty acids such as oleic, linoleic, eicosatetraynoic or eicosapentaenoic acids. PGHS-2 expression induced by AA application was independent of PGHS and lipoxygenase metabolite synthesis. However, topical application of PGE2 on skin induced PGHS-2 overexpression. This study suggests that AA release and/or subsequent metabolism by PGHS may be involved in the induction of PGHS-2 expression in murine TPA- and AA-induced ear oedema.

Alternate JournalProstaglandins Other Lipid Mediat
PubMed ID10410383
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