|Title||Mouse model of colorectal cancer: orthotopic co-implantation of tumor and stroma cells in cecum and rectum.|
|Publication Type||Journal Article|
|Year of Publication||2021|
|Authors||Kasashima H, Duran A, Cid-Diaz T, Muta Y, Kinoshita H, Batlle E, Diaz-Meco MT, Moscat J|
|Date Published||2021 Mar 19|
interrogation of the functional role of genes implicated in colorectal cancer (CRC) is limited by the need for physiological models that mimic the disease. Here, we describe a protocol that provides the steps required for the orthotopic co-implantation of tumoral and stromal cells into the cecum and rectum to investigate the crosstalk between the tumor and its microenvironment. This protocol recapitulates metastases to the lymph nodes, liver, and lungs observed in human CRC. For complete details on the use and execution of this protocol, please refer to Kasashima et al. (2020).
|Alternate Journal||STAR Protoc|
|PubMed Central ID||PMC7843928|
|Grant List||R01 CA207177 / CA / NCI NIH HHS / United States |
R01 CA211794 / CA / NCI NIH HHS / United States
R01 CA218254 / CA / NCI NIH HHS / United States
R01 DK108743 / DK / NIDDK NIH HHS / United States
Jorge Moscat, Ph.D. Maria Diaz-Meco Conde, Ph.D.