Monoclonal antibody OPD4 detects neoplastic T cells but does not distinguish between CD4 and CD8 neoplastic T cells in paraffin tissue sections.

TitleMonoclonal antibody OPD4 detects neoplastic T cells but does not distinguish between CD4 and CD8 neoplastic T cells in paraffin tissue sections.
Publication TypeJournal Article
Year of Publication1992
AuthorsChadburn A, Husain S, Knowles DM
JournalHum Pathol
Date Published1992 Aug
KeywordsAntibodies, Monoclonal, Antigens, CD, Antigens, Differentiation, T-Lymphocyte, CD3 Complex, CD4-Positive T-Lymphocytes, CD8 Antigens, Histocompatibility Antigens, Humans, Immunohistochemistry, Immunophenotyping, Leukocyte Common Antigens, Lymphoma, T-Cell, Paraffin, Receptors, Antigen, T-Cell, T-Lymphocyte Subsets, T-Lymphocytes

Monoclonal antibody (MoAb) OPD4, reported to preferentially react with benign CD4 T cells in formalin-fixed tissue sections, was examined for its reactivity with 56 T-cell neoplasms after formalin or Bouin's fixation to determine if it also preferentially detects neoplastic CD4 T cells in paraffin tissue sections. Monoclonal antibody OPD4 did not preferentially detect neoplastic CD4 T cells, since it reacted with 22 of 38 (58%) CD4-positive compared with nine of 14 (64%) CD4-negative T-cell neoplasms. However, MoAb OPD4 appears to detect neoplastic T cells in Bouin's-fixed (11 of 20 cases [55%]) about as well as in formalin-fixed (20 of 32 cases [63%]) tissues. Since MoAb OPD4 does not preferentially react with neoplastic CD4 T cells, the utility of this MoAb as a pan-T-cell marker in routinely processed tissues was also explored and compared with that of Leu-22, UCHL-1, and CD3. All four antibodies reacted with approximately the same percentage of T-cell malignancies (51% to 57%). However, examination of different clinicopathologic groups and types of fixative highlighted differences. Monoclonal antibodies OPD4 and Leu-22 reacted with 62%, while CD3 detected only 41% of formalin-fixed, postthymic T-cell neoplasms. OPD4, UCHL-1, and CD3 each reacted with 55%, but Leu-22 recognized only 45% of Bouin's-fixed, postthymic T-cell malignancies. OPD4 reacted with none, but CD3 reacted with all four T-cell lymphoblastic lymphomas. Various antibody combinations were examined to determine an optimal panel for the recognition of T-cell neoplasms in paraffin sections. The combination of MoAbs OPD4 and Leu-22 detected 86% of postthymic T-cell neoplasms in formalin-fixed tissue sections. Furthermore, MoAb OPD4 appears to be relatively specific for T-cell neoplasms, detecting 31 of 56 (55%) T-cell malignancies, while only reacting with two of 39 (5%) B-cell neoplasms. Therefore, while not preferentially reactive with neoplastic CD4 T cells, MoAb OPD4 may be useful as a pan-T-cell marker of postthymic T-cell neoplasms in routinely processed, formalin-fixed tissues, especially when used in conjunction with MoAb Leu-22.

Alternate JournalHum Pathol
PubMed ID1353748
Grant ListCA42836 / CA / NCI NIH HHS / United States
EY06337 / EY / NEI NIH HHS / United States
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