|Title||Immunoglobulin VH gene expression in human B cell lines and tumors: biased VH gene expression in chronic lymphocytic leukemia.|
|Publication Type||Journal Article|
|Year of Publication||1989|
|Authors||Logtenberg T, Schutte ME, Inghirami G, Berman JE, Gmelig-Meyling FH, Insel RA, Knowles DM, Alt FW|
|Keywords||Alleles, B-Lymphocytes, Cell Line, Transformed, Gene Expression, Genes, Immunoglobulin, Herpesvirus 4, Human, Humans, Leukemia, Lymphocytic, Chronic, B-Cell, Multigene Family|
We have studied frequencies of VH gene utilization in a panel of monoclonal Epstein-Barr virus (EBV)-transformed B cell lines derived from human adult and fetal tissues as well as in monoclonal B cells obtained from fresh chronic lymphocytic leukemia (CLL) samples. The results show that IgM-secreting EBV cell lines from both fetal and adult tissues utilize VH genes from particular families roughly in proportion to estimated family size, suggesting that the repertoire of sigM-positive B cells in both fetal and adult organs is 'normalized' with respect to the V(H) gene family. In contrast, we find a highly biased pattern of VH gene expression in CLLs. The significance of these findings is discussed in the context of mechanisms that could be involved in normal B cell repertoire development and in the process of malignant transformation of precursors of CLL.
|Alternate Journal||Int Immunol|
|Grant List||AI-200047 / AI / NIAID NIH HHS / United States |
CA48236 / CA / NCI NIH HHS / United States
EY06337 / EY / NEI NIH HHS / United States
Giorgio Inghirami, M.D.