|Title||Human lymphocytes making rheumatoid factor and antibody to ssDNA belong to Leu-1+ B-cell subset.|
|Publication Type||Journal Article|
|Year of Publication||1987|
|Authors||Casali P, Burastero SE, Nakamura M, Inghirami G, Notkins AL|
|Date Published||1987 Apr 03|
|Keywords||Antibodies, Anti-Idiotypic, Antibodies, Antinuclear, Antibodies, Bacterial, Antigens, Differentiation, B-Lymphocyte, Antigens, Differentiation, T-Lymphocyte, Antigens, Surface, Autoantibodies, B-Lymphocytes, Cell Separation, DNA, Single-Stranded, Flow Cytometry, Humans, Immunoglobulin A, Immunoglobulin D, Immunoglobulin Fc Fragments, Immunoglobulin G, Immunoglobulin M, Leukocyte Count, Rheumatoid Factor, Tetanus Toxoid|
B lymphocytes bearing the Leu-1 cell-surface antigen (Leu-1+), the human equivalent of mouse Ly-1+ B lymphocytes, have been detected in human peripheral blood, but there is little information on their frequency and properties. Analysis by fluorescence-activated cell sorter and double immunofluorescence showed that Leu-1+ B cells are consistently present in the peripheral blood and spleens of healthy subjects and constitute 17.0 +/- 5.0% (mean value +/- standard deviation) and 17.3 +/- 3.9%, respectively, of total B cells. When purified Leu-1+ and Leu-1- B lymphocytes were transformed into immunoglobulin-secreting cells by infection with Epstein-Barr virus and the culture fluids were tested for reactivity with self-antigens, at least two important autoantibodies, antibody to the Fc fragment of human immunoglobulin G (rheumatoid factor) and antibody to single-stranded DNA, were found to be made exclusively by Leu-1+ B cells. It is concluded that the Leu-1+ lymphocytes represent a major subset of the normal human B cell repertoire and include the B cells capable of making autoantibodies similar to those found in systemic lupus erythematosus and rheumatoid arthritis.
Giorgio Inghirami, M.D.