Germinal center B cells regulate their capability to present antigen by modulation of HLA-DO.

TitleGerminal center B cells regulate their capability to present antigen by modulation of HLA-DO.
Publication TypeJournal Article
Year of Publication2002
AuthorsGlazier KS, Hake SB, Tobin HM, Chadburn A, Schattner EJ, Denzin LK
JournalJ Exp Med
Volume195
Issue8
Pagination1063-9
Date Published2002 Apr 15
ISSN0022-1007
KeywordsAntigen Presentation, Antigen-Presenting Cells, Antigens, Differentiation, B-Lymphocyte, B-Lymphocyte Subsets, B-Lymphocytes, Cell Line, Down-Regulation, Germinal Center, Histocompatibility Antigens Class II, HLA-D Antigens, HLA-DR Antigens, Humans
Abstract

Peptide acquisition by MHC class II molecules is catalyzed by HLA-DM (DM). In B cells, HLA-DO (DO) inhibits or modifies the peptide exchange activity of DM. We show here that DO protein levels are modulated during B cell differentiation. Remarkably, germinal center (GC) B cells, which have low levels of DO relative to naive and memory B cells, are shown to have enhanced antigen presentation capabilities. DM protein levels also were somewhat reduced in GC B cells; however, the ratio of DM to DO in GC B cells was substantially increased, resulting in more free DM in GC B cells. We conclude that modulation of DM and DO in distinct stages of B cell differentiation represents a mechanism by which B cells regulate their capacity to function as antigen-presenting cells. Efficient antigen presentation in GC B cells would promote GC B cell-T cell interactions that are essential for B cells to survive positive selection in the GC.

DOI10.1084/jem.20012059
Alternate JournalJ Exp Med
PubMed ID11956297
PubMed Central IDPMC2193692
Grant ListP30 CA008748 / CA / NCI NIH HHS / United States
R01 AI046202 / AI / NIAID NIH HHS / United States
AI46202 / AI / NIAID NIH HHS / United States
P30-CA08748 / CA / NCI NIH HHS / United States
Related Faculty: 
Amy Chadburn, M.D.

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