Detection of TCR-gamma gene rearrangements in early mycosis fungoides by non-radioactive PCR-SSCP.

TitleDetection of TCR-gamma gene rearrangements in early mycosis fungoides by non-radioactive PCR-SSCP.
Publication TypeJournal Article
Year of Publication2000
AuthorsMurphy M, Signoretti S, Kadin ME, Loda M
JournalJ Cutan Pathol
Date Published2000 May
KeywordsBiopsy, DNA Primers, DNA, Neoplasm, Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor, Humans, Mycosis Fungoides, Paraffin Embedding, Polymerase Chain Reaction, Polymorphism, Single-Stranded Conformational, Receptors, Antigen, T-Cell, gamma-delta, Recurrence, Skin Neoplasms

Early mycosis fungoides (MF) can mimic numerous benign inflammatory dermatoses on routine histological examination. In this study, a recently developed non-radioactive polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP) technique was used to assess T-cell clonality in paraffin-embedded skin biopsies clinically and pathologically suspicious for early MF. Non-radioactive PCR-SSCP is a simple, sensitive, reproducible and rapid procedure requiring minimal instrumentation. DNA was extracted from 22 skin biopsies of 20 patients with suspected patch stage MF and 15 skin biopsies of inflammatory dermatoses. V gamma1-8, V gamma9, V gamma10, V gamma11 and J gamma1/J gamma2 consensus primers were used for T-cell receptor (TCR)-gamma gene rearrangement amplification. PCR products were analyzed by non-radioactive SSCP. Clonal TCR-gamma gene rearrangements were detected in 17 of 22 (77%) suspected MF specimens. Clonal SSCP banding patterns were different among individual patients. In addition, identical banded patterns were demonstrated in serial skin biopsies from the same patient. No dominant T-cell clones were found in the inflammatory dermatoses studied. Therefore, non-radioactive PCR-SSCP is a useful molecular diagnostic tool for assessment of T-cell clonality in paraffin-embedded specimens suspicious for early MF. The SSCP imprint of PCR products is specific for each TCR-gamma gene rearrangement, and may be used to evaluate concurrent/recurrent disease in individual patients.

Alternate JournalJ Cutan Pathol
PubMed ID10847547
Related Faculty: 
Massimo Loda, M.D.

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