A conserved downstream element defines a new class of RNA polymerase II promoters.

TitleA conserved downstream element defines a new class of RNA polymerase II promoters.
Publication TypeJournal Article
Year of Publication1995
AuthorsInce TA, Scotto KW
JournalJ Biol Chem
Date Published1995 Dec 22
KeywordsAnimals, ATP Binding Cassette Transporter, Subfamily B, Member 1, Base Sequence, Binding Sites, Cell Line, Conserved Sequence, Humans, Hydroxymethylglutaryl CoA Reductases, Hypoxanthine Phosphoribosyltransferase, Luciferases, Molecular Sequence Data, Mutagenesis, Site-Directed, Oligodeoxyribonucleotides, Promoter Regions, Genetic, Recombinant Proteins, Regulatory Sequences, Nucleic Acid, RNA Polymerase II, Sequence Homology, Nucleic Acid, TATA Box, Thymidine Kinase, Thymidylate Synthase, Transcription, Genetic, Transfection

Although many TATA-less promoters transcribed by RNA polymerase II initiate transcription at multiple sites, the regulation of multiple start site utilization is not understood. Beginning with the prediction that multiple start site promoters may share regulatory features and using the P-glycoprotein promoter (which can utilize either a single or multiple transcription start site(s)) as a model, several promoters with analogous transcription windows were grouped and searched for the presence of a common DNA element. A downstream protein-binding sequence, MED-1 (Multiple start site Element Downstream), was found in the majority of promoters analyzed. Mutation of this element within the P-glycoprotein promoter reduced transcription by selectively decreasing utilization of downstream start sites. We propose that a new class of RNA polymerase II promoters, those that can utilize a distinctive window of multiple start sites, is defined by the presence of a downstream MED-1 element.

Alternate JournalJ Biol Chem
PubMed ID8530439
Grant ListP30-CA-08748 / CA / NCI NIH HHS / United States
R01-CA57307 / CA / NCI NIH HHS / United States
Related Faculty: 
Tan Ince, M.D., Ph.D.

Pathology & Laboratory Medicine 1300 York Avenue New York, NY 10065 Phone: (212) 746-6464
Surgical Pathology: (212) 746-2700