Comparative genomics reveals the correlations of stress response genes and bacteriophages in developing antibiotic resistance of Staphylococcus saprophyticus.

TitleComparative genomics reveals the correlations of stress response genes and bacteriophages in developing antibiotic resistance of Staphylococcus saprophyticus.
Publication TypeJournal Article
Year of Publication2023
AuthorsZhang K, Potter RF, Marino J, Muenks CE, Lammers MG, Bard JDien, Dingle TC, Humphries R, Westblade LF, Burnham C-AD, Dantas G
JournalmSystems
Volume8
Issue6
Paginatione0069723
Date Published2023 Dec 21
ISSN2379-5077
KeywordsAnti-Bacterial Agents, Drug Resistance, Microbial, Female, Genomics, Humans, Staphylococcus saprophyticus, Trimethoprim, Sulfamethoxazole Drug Combination, Urinary Tract Infections
Abstract

Staphylococcus saprophyticus is the second most common bacteria associated with urinary tract infections (UTIs) in women. The antimicrobial treatment regimen for uncomplicated UTI is normally nitrofurantoin, trimethoprim-sulfamethoxazole (TMP-SMX), or a fluoroquinolone without routine susceptibility testing of S. saprophyticus recovered from urine specimens. However, TMP-SMX-resistant S. saprophyticus has been detected recently in UTI patients, as well as in our cohort. Herein, we investigated the understudied resistance patterns of this pathogenic species by linking genomic antibiotic resistance gene (ARG) content to susceptibility phenotypes. We describe ARG associations with known and novel SCCmec configurations as well as phage elements in S. saprophyticus, which may serve as intervention or diagnostic targets to limit resistance transmission. Our analyses yielded a comprehensive database of phenotypic data associated with the ARG sequence in clinical S. saprophyticus isolates, which will be crucial for resistance surveillance and prediction to enable precise diagnosis and effective treatment of S. saprophyticus UTIs.

DOI10.1128/msystems.00697-23
Alternate JournalmSystems
PubMed ID38051037
PubMed Central IDPMC10734486
Grant ListR01 AI155893 / AI / NIAID NIH HHS / United States
U01 AI123394 / AI / NIAID NIH HHS / United States
R01 HS027621 / HS / AHRQ HHS / United States
Related Faculty: 
Jamie Marino, Ph.D.

Pathology & Laboratory Medicine 1300 York Avenue New York, NY 10065 Phone: (212) 746-6464
Surgical Pathology: (212) 746-2700