Blocking PI3K p110β Attenuates Development of PTEN-Deficient Castration-Resistant Prostate Cancer.

TitleBlocking PI3K p110β Attenuates Development of PTEN-Deficient Castration-Resistant Prostate Cancer.
Publication TypeJournal Article
Year of Publication2022
AuthorsGao X, Wang Y, Ribeiro CF, Manokaran C, Chang H, Von T, Rodrigues S, Cizmecioglu O, Jia S, Korpal M, Korn JM, Wang Z, Schmit F, Jiang L, Pagliarini R, Yang Y, Sethi I, Signoretti S, Yuan G-C, Loda M, Zhao JJ, Roberts TM
JournalMol Cancer Res
Date Published2022 05 04
KeywordsAndrogen Antagonists, Animals, Humans, Male, Mice, Phosphatidylinositol 3-Kinases, Prostate, Prostatic Neoplasms, Castration-Resistant, PTEN Phosphohydrolase, Tankyrases

A common outcome of androgen deprivation in prostate cancer therapy is disease relapse and progression to castration-resistant prostate cancer (CRPC) via multiple mechanisms. To gain insight into the recent clinical findings that highlighted genomic alterations leading to hyperactivation of PI3K, we examined the roles of the commonly expressed p110 catalytic isoforms of PI3K in a murine model of Pten-null invasive CRPC. While blocking p110α had negligible effects in the development of Pten-null invasive CRPC, either genetic or pharmacologic perturbation of p110β dramatically slowed CRPC initiation and progression. Once fully established, CRPC tumors became partially resistant to p110β inhibition, indicating the acquisition of new dependencies. Driven by our genomic analyses highlighting potential roles for the p110β/RAC/PAK1 and β-catenin pathways in CRPC, we found that combining p110β with RAC/PAK1 or tankyrase inhibitors significantly reduced the growth of murine and human CRPC organoids in vitro and in vivo. Because p110β activity is dispensable for most physiologic processes, our studies support novel therapeutic strategies both for preventing disease progression into CRPC and for treating CRPC.

IMPLICATIONS: This work establishes p110β as a promising target for preventing the progression of primary PTEN-deficient prostate tumors to CRPC, and for treating established CRPC in combination with RAC/PAK1 or tankyrase inhibitors.

Alternate JournalMol Cancer Res
PubMed ID35105671
PubMed Central IDPMC9081176
Grant ListR01 CA187918 / CA / NCI NIH HHS / United States
R35 CA231945 / CA / NCI NIH HHS / United States
R35 CA210057 / CA / NCI NIH HHS / United States
S10 OD026880 / OD / NIH HHS / United States
S10 OD030463 / OD / NIH HHS / United States
Related Faculty: 
Massimo Loda, M.D.

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