Title | Rapid isolation and identification of bacteriophage T4-encoded modifications of Escherichia coli RNA polymerase: a generic method to study bacteriophage/host interactions. |
Publication Type | Journal Article |
Year of Publication | 2008 |
Authors | Westblade LF, Minakhin L, Kuznedelov K, Tackett AJ, Chang EJ, Mooney RA, Vvedenskaya I, Wang QJun, Fenyƶ D, Rout MP, Landick R, Chait BT, Severinov K, Darst SA |
Journal | J Proteome Res |
Volume | 7 |
Issue | 3 |
Pagination | 1244-50 |
Date Published | 2008 Mar |
ISSN | 1535-3893 |
Keywords | Amino Acid Sequence, Bacteriophage T4, DNA-Directed RNA Polymerases, Electrophoresis, Polyacrylamide Gel, Escherichia coli, Mass Spectrometry, Molecular Sequence Data, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization |
Abstract | Bacteriophages are bacterial viruses that infect bacterial cells, and they have developed ingenious mechanisms to modify the bacterial RNA polymerase. Using a rapid, specific, single-step affinity isolation procedure to purify Escherichia coli RNA polymerase from bacteriophage T4-infected cells, we have identified bacteriophage T4-dependent modifications of the host RNA polymerase. We suggest that this methodology is broadly applicable for the identification of bacteriophage-dependent alterations of the host synthesis machinery. |
DOI | 10.1021/pr070451j |
Alternate Journal | J Proteome Res |
PubMed ID | 18271525 |
Grant List | GM59295 / GM / NIGMS NIH HHS / United States GM61898 / GM / NIGMS NIH HHS / United States RR00862 / RR / NCRR NIH HHS / United States |
Related Faculty:
Lars Westblade, Ph.D.