Title | Loss of PKC lambda/iota impairs Th2 establishment and allergic airway inflammation in vivo. |
Publication Type | Journal Article |
Year of Publication | 2009 |
Authors | Yang J-Q, Leitges M, Duran A, Diaz-Meco MT, Moscat J |
Journal | Proc Natl Acad Sci U S A |
Volume | 106 |
Issue | 4 |
Pagination | 1099-104 |
Date Published | 2009 Jan 27 |
ISSN | 1091-6490 |
Keywords | Animals, Cell Differentiation, Cell Polarity, Cell Proliferation, Cytokines, Hypersensitivity, Immunoglobulin E, Inflammation, Isoenzymes, Lymphocyte Activation, Mice, Mice, Knockout, Ovalbumin, Protein Kinase C, Respiratory System, Th2 Cells, Transcription Factors, Up-Regulation |
Abstract | The differentiation of T cells along different lineages is central to the control of immunity. Here we have used a conditional gene knockout system to delete PKC lambda/iota selectively in activated T cells. With this system we have demonstrated that PKC lambda/iota is necessary for T-helper cell (Th2) cytokine production and optimal T-cell proliferation and allergic airway inflammation in vivo. Our data demonstrate that the activation of the transcription factors nuclear factor of activated T cells and NF-kappaB is impaired in PKC lambda/iota-deficient activated T cells. In addition, we present genetic knockout evidence in ex vivo experiments with primary T cells that PKC lambda/iota is critical for the control of cell polarity during T-cell activation. Therefore PKC lambda/iota emerges as a critical regulator of Th 2 activation. |
DOI | 10.1073/pnas.0805907106 |
Alternate Journal | Proc Natl Acad Sci U S A |
PubMed ID | 19144923 |
Grant List | R01-AI072581 / AI / NIAID NIH HHS / United States |
Related Faculty:
Jorge Moscat, Ph.D. Maria Diaz-Meco Conde, Ph.D.