A transcription activator with restricted tissue distribution regulates cell-specific expression of alpha1(XI) collagen.

TitleA transcription activator with restricted tissue distribution regulates cell-specific expression of alpha1(XI) collagen.
Publication TypeJournal Article
Year of Publication1997
AuthorsKinoshita A, Greenwel P, Tanaka S, Di Liberto M, Yoshioka H, Ramirez F
JournalJ Biol Chem
Volume272
Issue50
Pagination31777-84
Date Published1997 Dec 12
ISSN0021-9258
Keywords3T3 Cells, Adult, Animals, Base Sequence, Binding Sites, Cell Differentiation, Collagen, Humans, Jurkat Cells, Mice, Mice, Inbred C3H, Molecular Sequence Data, Muscle, Skeletal, Osteoblasts, Peptide Fragments, Promoter Regions, Genetic, Tissue Distribution, Trans-Activators, Tumor Cells, Cultured
Abstract

Different regulatory programs are likely to control expression of the alpha1(XI) collagen (COL11A1) gene in cartilaginous and non-cartilaginous tissues and in coordination with different collagen genes. Here, we report the identification of a cis-acting element that is required for constitutive and tissue-specific activity of the proximal COL11A1 promoter. The element binds an apparently novel activator whose expression is restricted mostly, but not exclusively, to cells of mesenchymal origin. Transient transfection experiments using wild-type and mutant constructs demonstrated the critical contribution of a 45-base pair upstream element (FP9) to promoter activity. The same functional tests and DNA binding assays narrowed down the critical portion of FP9 to a 20-base pair sequence, which consists of an imperfect palindrome with strong homology to the GATA consensus motif. Despite being able to bind GATA proteins in vitro, FP9 is actually recognized by a distinct approximately 100-kDa polypeptide (FP9C) probably belonging to the zinc-finger family of transcription factors. FP9C binding was mostly identified in nuclei from cells of mesenchymal origin, including those actively engaged in COL11A1 transcription. A positive correlation was also established between the level of FP9C binding and the degree of cell differentiation in vitro. Thus, FP9C represents an unusual example of tissue-specific and differentiation-related transcription factor with overlapping expression in hard and soft connective tissues.

DOI10.1074/jbc.272.50.31777
Alternate JournalJ Biol Chem
PubMed ID9395523
Grant ListR37-38648 / / PHS HHS / United States
Related Faculty: 
Maurizio DiLiberto, Ph.D.

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