The human "peripheral-type" benzodiazepine receptor: regional mapping of the gene and characterization of the receptor expressed from cDNA.

TitleThe human "peripheral-type" benzodiazepine receptor: regional mapping of the gene and characterization of the receptor expressed from cDNA.
Publication TypeJournal Article
Year of Publication1992
AuthorsChang YJ, McCabe RT, Rennert H, Budarf ML, Sayegh R, Emanuel BS, Skolnick P, Strauss JF
JournalDNA Cell Biol
Volume11
Issue6
Pagination471-80
Date Published1992 Jul-Aug
ISSN1044-5498
KeywordsBase Sequence, Blotting, Northern, Cell Line, Chromosome Mapping, Chromosomes, Human, Pair 22, Cloning, Molecular, DNA, Humans, Liver, Molecular Sequence Data, Polymerase Chain Reaction, Receptors, GABA-A
Abstract

A cDNA for the human "peripheral-type" benzodiazepine receptor (PBR) was isolated from a liver cDNA library. The 851-nucleotide probe hybridized with a approximately 1 kb mRNA in Northern blots of RNA extracted from various human tissues and cell lines. The human PBR probe was hybridized to DNA from a somatic cell hybrid mapping panel to determine that the gene maps to chromosome 22. With a regional mapping panel for chromosome 22, we localized the gene within band 22q13.31. The ligand-binding properties of the receptor expressed from the cDNA were examined in transient expression experiments and compared to the endogenous human PBR. The PBR ligand [3H]PK 11195 had high affinity for the expressed receptor in COS-1 cells, but the affinities of a pair of isoquinoline propanamide enantiomers differed remarkably in expressed and endogenous human PBR. These findings reveal that the host cell and/or post-translational modification may have an important influence on PBR function.

DOI10.1089/dna.1992.11.471
Alternate JournalDNA Cell Biol
PubMed ID1326278
Grant ListCA-39926 / CA / NCI NIH HHS / United States
HD-06274 / HD / NICHD NIH HHS / United States
HG-00425 / HG / NHGRI NIH HHS / United States
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