Title | The human "peripheral-type" benzodiazepine receptor: regional mapping of the gene and characterization of the receptor expressed from cDNA. |
Publication Type | Journal Article |
Year of Publication | 1992 |
Authors | Chang YJ, McCabe RT, Rennert H, Budarf ML, Sayegh R, Emanuel BS, Skolnick P, Strauss JF |
Journal | DNA Cell Biol |
Volume | 11 |
Issue | 6 |
Pagination | 471-80 |
Date Published | 1992 Jul-Aug |
ISSN | 1044-5498 |
Keywords | Base Sequence, Blotting, Northern, Cell Line, Chromosome Mapping, Chromosomes, Human, Pair 22, Cloning, Molecular, DNA, Humans, Liver, Molecular Sequence Data, Polymerase Chain Reaction, Receptors, GABA-A |
Abstract | A cDNA for the human "peripheral-type" benzodiazepine receptor (PBR) was isolated from a liver cDNA library. The 851-nucleotide probe hybridized with a approximately 1 kb mRNA in Northern blots of RNA extracted from various human tissues and cell lines. The human PBR probe was hybridized to DNA from a somatic cell hybrid mapping panel to determine that the gene maps to chromosome 22. With a regional mapping panel for chromosome 22, we localized the gene within band 22q13.31. The ligand-binding properties of the receptor expressed from the cDNA were examined in transient expression experiments and compared to the endogenous human PBR. The PBR ligand [3H]PK 11195 had high affinity for the expressed receptor in COS-1 cells, but the affinities of a pair of isoquinoline propanamide enantiomers differed remarkably in expressed and endogenous human PBR. These findings reveal that the host cell and/or post-translational modification may have an important influence on PBR function. |
DOI | 10.1089/dna.1992.11.471 |
Alternate Journal | DNA Cell Biol |
PubMed ID | 1326278 |
Grant List | CA-39926 / CA / NCI NIH HHS / United States HD-06274 / HD / NICHD NIH HHS / United States HG-00425 / HG / NHGRI NIH HHS / United States |
Related Faculty:
Hanna Rennert, Ph.D.