Evidence for early hematopoietic progenitor cell involvement in acute promyelocytic leukemia.

TitleEvidence for early hematopoietic progenitor cell involvement in acute promyelocytic leukemia.
Publication TypeJournal Article
Year of Publication1999
AuthorsEdwards RH, Wasik MA, Finan J, Rodriguez R, Moore J, Kamoun M, Rennert H, Bird J, Nowell PC, Salhany KE
JournalAm J Clin Pathol
Date Published1999 Dec
KeywordsAntigens, CD34, Antineoplastic Agents, Chromosomes, Human, Pair 15, Chromosomes, Human, Pair 17, Flow Cytometry, Hematopoietic Stem Cells, HLA-DR Antigens, Humans, Immunophenotyping, Karyotyping, Leukemia, Promyelocytic, Acute, Reverse Transcriptase Polymerase Chain Reaction, Translocation, Genetic, Tretinoin

Acute promyelocytic leukemia (APL) represents a subtype of acute myeloid leukemia with characteristic morphologic, molecular, and immunophenotypic features. Previous immunophenotypic analyses have shown that leukemic cells in APL typically express the myeloid markers CD33 and CD13 but lack expression of the early hematopoietic progenitor cell antigens CD34 and HLA-DR. We analyzed selected immunophenotypic features of APL by flow cytometry and showed that 7 (41%) of 17 cases contained significant subsets of CD34+ leukemic cells: CD34+ myeloid cells predominated in 2 APL cases. By using a fluorescence-activated cell sorter-fluorescence in situ hybridization approach, we confirmed that the CD34+ cells harbored the t(15;17) translocation characteristic of APL. By using the same experimental approach, CD34+ populations were stratified into primitive CD34+ CD38- and committed CD34+ CD38+ progenitor cell subpopulations; cells in both subsets contained the t(15;17) translocation. The knowledge that APL may be partly or largely CD34+ is important for proper diagnosis. Furthermore, identification of the t(15;17) translocation in CD34+ CD38- blasts indicates that, in at least some cases, the leukemogenic mutation in APL occurs within primitive hematopoietic progenitor cells.

Alternate JournalAm J Clin Pathol
PubMed ID10587705
Grant ListCA 42232 / CA / NCI NIH HHS / United States
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