The Translational Research Program (TRP) is a resource funded by the Department of Pathology and Laboratory Medicine at NewYork-Presbyterian/Weill Cornell Medical Center. The TRP logs submitted projects into a database, tracks requests, monitors research integrity, and lists publications resulting from these investigations.
Three laboratories are directed by the TRP: the Histology Laboratory, Immunohistochemistry Laboratory, and Molecular Laboratory. The three laboratories collectively provide services to perform a wide range of assays, including:
- Fluorescence in situ hybridization (FISH).
- Immunohistochemistical (IHC) staining.
- High-throughput expression profiling (i.e. Biotrove, Illumina).
- Micro-RNA expression analysis (i.e., Luminex).
- Tissue microarray (TMA) construction.
- Routine histology.
The TRP also provides statistical support for study design, data analysis, and publication writing. The utility of the TRP is grounded in its accessibility of archival and frozen material from within the Pathology and Laboratory Medicine Department. All TRP facilities are located in close proximity to each other in the Starr Pavilion and in the F Building of Weill Cornell Medicine, making them easily accessible to investigators.
The Leica Bond Autostainer is capable of running multiple IHC protocols simultaneously, with or without routine de-paraffination and pretreatment. Protocols are 100-percent customizable, and multiple chromagens can be used side by side. The automation of the system provides highly specific and reproducible results. Recent upgrades to this equipment now allow for double staining and triple staining within the same protocol to examine co-expression.
The Ariol system is a high-throughput imaging platform that allows multiple tissue microarrays and conventional slides to be scored objectively with the same criteria. Slides scanned into the Ariol system can then be classified based on subcellular features and analyzed for immunoperoxidase staining intensity to generate quantitative data.